DenardResearchGroup @CarlDenard
Assist. Prof. of Chem. Eng. Enzyme Therapeutics, Synthetic Biology, Protein Engineering and Design. https://t.co/3RUXqR9nXX Soccer player. Music lover. University of Florida-Gainesvi Joined November 2019-
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These GPVs would be a significant advance in synthetic cellular mimetics. This work comes from our ongoing collaboration with Yeongseon Jang.
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He developed cloning methods to produce different architectures of these protein fusions. He then showed these GPV containing ELP bolaamphiphiles had different biophysical properties. This paper lays the foundation for generating GPVs with transmembrane-like signal transductions.
We start 2026 strong with a publication in ChemSystemsChem. Luis and coworkers wanted to determine whether globular protein vesicles could be formed using ELP bolaamphiphiles, i.e., ELPs flanked by soluble globular proteins at both ends. lnkd.in/eBmBgWxY
HARP enables the discovery of protease-inhibitory nanobodies from large synthetic libraries, bypassing the more numerous non-inhibitory binders. pubs.acs.org/doi/10.1021/ac…
After a few months in review, HARP is finally out! Congrats to Samantha Martinusen, Ph.D., Ethan Slaton, and Nima Ajayebi for leading experimental and computational arms. Special thanks to Amit Dutta and Steven Bruner for the structural analysis.
Congratulations to Sage Nelson for being one of the four graduate students who received the Excellence in Research Award from the UF Department of Chemical Engineering. Sage is now a fourth year graduate student, has already authored several papers. Amazing scientist!
Hello Xverse, if anyone has a plasmid to express and purify IdeS from E. coli, please send me a DM.
@jenheemstra @Harvard_Press I pre-ordered.
DenardResearchGroup retweeted
Online now @ Cell is the yeast multicellular engineering paper from @DrFankangMeng - the fruits of his productive PhD. He developed modular synthetic biology tools to bring multicellular behaviours to yeast - adhesion, juxtacrine signalling and more. cell.com/cell/fulltext/…
TwitterWorld, my lab wants to get into some optogenetics work (bacteria, yeast, in vitro). Are there good protocols to build cheap systems out there?
DenardResearchGroup retweeted
@ATinyGreenCell when will this be added to your catalog? i want it.
DenardResearchGroup retweeted
Excited to share the Hanson Lab’s @ADHansonLab new preprint! We used OrthoRep to evolve Arabidopsis HDH, a short-lived enzyme, selecting variants with up to 20× higher abundance. Mutations boosted lifespan, catalytic efficiency, or inhibitor resistance. 🔗doi.org/10.1101/2025.0…
Has anyone made RNA-triggered toehold switches that work in S. cerevisiae? If you know of some good papers, let me know.
This work lays the foundation for the bottom-up construction of GPVs with transmembrane signal transduction capabilities. Go Team!
New PrePrint from Denard Lab: biorxiv.org/content/10.110… In this collaboration with the Jang lab, we designed recombinant ELP bolaamphiphiles to construct multifunctional globular protein vesicles.
"New" Paper Alert from a collab with Jang Lab. We designed and characterized globular protein vesicles with ability to sense small molecules in their surroundings. Congrats to her awesome students. pubs.acs.org/doi/abs/10.102…
Sage Nelson is the driver of this work, with tremendous help from Jokent Gaza, Ron Masse, and others. Alberto Perez and Amor Menezes collaborators on this work.
pubs.acs.org/doi/10.1021/ac… PERRC is now out. Last month, we presented this work on manipulating ER retention signals to modulate counterselection to selection substrate ratios in the yeast ER and how we used it for protease engineering.
Happy to share an authoritative, topical, and detailed review paper on cover all the latest efforts to engineer proteases, out now in Biotech Avdances. sciencedirect.com/science/articl… Led by @sammartinusen, with considerable efforts from many lab members.
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