Dylan Burnette @MAG2ART
A quantitative cell biologist who studies how force-generating cytoskeletal systems organize biological function across scales. Married to @gillianhoo. lab.vanderbilt.edu/dylan-burnette… Nashville, TN Joined January 2015-
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Mitochondria in a crawling fibroblast cell videoed through a spinning disk confocal microscope by Burnette Lab member, Atsuko Price. #CellBiology
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@JovieOnandon 使用 Photoshop 更改了每个细胞细胞核的颜色。
Nuclei in cancer cells photographed through a microscope. DNA is shown. #CellBiology
Mitochondria in a living cell, imaged today while I was training new lab members on our spinning-disk confocal microscope. Shown are the full cell and zoomed-in regions of interest. Total imaging time: 60 minutes. #CellBiology
@XueyingWu_Mito The colors represent the relative axial (Z) height. The Z stack shows 5 microns from the substrate the cells are sitting on. The color look up table was "spectrum" which is the rainbow starting with red on the bottom (0 microns) and purple on the top (5 microns).
Mitochondria in two cells videoed through a spinning disk confocal microscope. #CellBiology
A control cell going through cell division with normal membrane blebs (top) and a cell we messed with (bottom). Both were videoed through a DIC microscope. #CellBiology molbiolcell.org/doi/full/10.10…
Not all cells do the whole membrane blebbing thing the same. Some of them get weird about it. This is a single cell videoed through a DIC microscope. #CellBiology
Actin filament-based structures in a cell photographed using structured illumination microscopy. The video starts with the bottom of the cell and steps up through the Z dimension (i.e., going up from the substrate). #CellBiology rupress.org/jcb/article/20…
@Tabdanov It is a video of a growth cone I acquired in grad school. While the video was not published it was part of a data set that was used measure MT dynamics in control growth cones. I would be happy for you to use it. Just let me know.
Microtubules (green) fighting retrograde actin flow (magenta) in a neuronal growth cone videoed through a TIRF microscope. #CellBiology
@Tabdanov I do not know but I look forward to finding out!
The newest episode of Nikon's Small World Speaks. "Dylan Burnette (that's me) shares some of the stories behind one of his favorite images he's submitted to the Nikon Small World competition to date." #CellBiology
@Tabdanov Many groups have since worked out the molecular details of the connection between F-actin and MTs. Personally, I got distracted by actomyosin contraction and never got back to microtubules. However, every so often we help out a collaborator.......... pubs.acs.org/doi/10.1021/ac…
This was my first journal cover from back in the day. It shows the side of a neuronal growth cone photographed with an electron microscope. The microtubules (green) were labeled with nanoscale particles of colloidal gold bound to antibodies (i.e., immunogold). #CellBiology
@Tabdanov I think the evidence is pretty strong that microtubules are physically bound to actin filaments. Half of my PhD thesis could be boiled down to simply, "Actin bundles in the neuronal growth cone move microtubules around for some reason." #CellBiology
Dylan Burnette retweeted
Sometimes blebbisomes like to hitch a ride on cells, like you see here! Extracellular vesicles with a personality indeed! These guys do so many cool and unique things that we will be sharing soon! Stay tuned!#fluorescencefriday #blebbisomes #extracellularvesicle
A dividing cell videoed through a microscope. Hot tip: Don't mess around with myosin II paralogs if you don't want your cells to freak out. #CellBiology cell.com/cell-reports/f…
The inside of a heart muscle cell photographed with an electron microscope. The powerhouses/overlords of the cell, mitochondria, are shown in pink. The repeating structures are the basic units of contraction that drive each heart beat (i.e., sarcomeres). #CellBiology
The heart of a zebrafish embryo photographed through a microscope. (Ventricle: Left; Atrium: Right). The nuclei of both the heart cells and blood cells are shown. "What? Red blood cells do not have nuclei!", you say. So human centric of you...... #CellBiology
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