Aaron Barnes @MicroTolo
Med Micro, public health, enterococci, biofilms, microbiome, microscopist, clinical pathology, #doubledoc #postdoc. All tweets are my opinions only. Minneapolis, MN Joined August 2009-
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@jdpereira @BethBGenetics Agree that qPCR is both useful and a good tool; disagree that RNAseq analysis is improved by use of a "gold standard" that is frequently more susceptible to bias (in many forms) than RNAseq itself. Reasonable minds may differ, depending on the exact nature of their question.
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@jdpereira @BethBGenetics I have two ways to measure the length of a room: a calibrated laser rangefinder and a warped wooden meter stick with half the markings rubbed off. I can use both methods to obtain an answer, but exactly how useful is the meter stick in this case?
@Randy_Au The Fine French Wine Fallacy: Q: If you take a barrel of fine French wine and add a cup of crap to it, what do you have? A: The same thing you'd have if you took a barrel of crap and added a cup of fine French wine. Once you add crap, it's all crap.
@rocza "Save us from able-bodied white men who just can't handle facts" fits on a T-shirt pretty well.🤔 Just saying.
Aaron Barnes retweeted
THERE’S NO REASON TO VALIDATE YOUR RNA-SEQ EXPERIMENT WITH qPCR. RNA-SEQ IS A FAIRLY UNBIASED APPROACH TO ASSESS TRANSCRIPT QUANTITY ACROSS THE WHOLE GENOME AND DOESN’T SUFFER FROM PROBE BIAS THAT CAN BE EXPERIENCED WITH QPCR PRIMERS
@Merz EXACTLY. "I'll take 'Things we already knew' for $500, please". 🤣
@S__Baksh @RiderToast Best advice I ever got in med school: "On the wards you can not eat, or you can not sleep, but you can't not eat AND not sleep."
@gingerest @ProfLikeSubst I think you misspelled "the U.S. healthcare 'system' is a Gordian Knot that often seems custom-made to drive good people into a rage when they try to understand it, use it, or even think about it for more than a few minutes at a time." 😉🙃
@textfiles The acquisition of wisdom is often temporarily uncomfortable. -- Lao-Tzu (probably)
@MarkHoofnagle Seems like a metaphor for *most* things these days...... 😬
@steubens7 @textfiles Username checks out.
@garysimonds6 That's excuse number .....five, right? Or six? 🙄
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