Alison Fanton @AlisonFanton

Scientist in the Hsu Lab, Arc Institute Joined March 2021

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Patrick Honma @PatrickHonma

8 months ago

Excited to share our new preprint! How do animals transform internal representations into step-by-step navigation to goals? Decoding these strategies directly from behavior is challenging because these internal states are not directly observable. biorxiv.org/content/10.110… 🧵1/n

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Alison Fanton @AlisonFanton

8 months ago

Thrilled to share our paper, now out in Nature Biotechnology! We engineered recombinases to enable efficient, specific genome insertion of multi-kb DNA cargoes at an endogenous locus. Grateful to all my amazing co-authors!

Arc Institute @arcinstitute

8 months ago

A study published today in @NatureBiotech from the @pdhsu lab presents a new way to insert large DNA sequences into the genome using engineered recombinases that don’t require DNA cutting or rely on the cell's repair machinery.

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Alison Fanton @AlisonFanton

2 years ago

@ntperry13 Thank you Nick!!! :’)

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Alison Fanton @AlisonFanton

2 years ago

@pdhsu Thank you Patrick!! It’s been a fun and exciting five years in your lab, thank so much for your support ☺️

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Alison Fanton @AlisonFanton

2 years ago

especially @BartieLiam and @pdhsu , for your tireless teamwork and support. It’s been a fun PhD!👩🏻‍🔬

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Alison Fanton @AlisonFanton

2 years ago

🧬 Thrilled to share our latest work on engineering precise DNA recombinases for targeted genome integration! Through directed evolution & protein fusions, we achieved over 50% efficiency & 97% specificity for multi-kb DNA cargo insertions. Big thanks to all co-authors -

Patrick Hsu @pdhsu

2 years ago

🧵In new work, we report a systematic engineering roadmap to optimize large serine recombinases (LSRs) for direct, site-specific insertion into the human genome 🧬. We achieved over 50% insertion efficiency and 97% genome-wide specificity, a 10X improvement over our previous work

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Sita Chandrasekaran @sitaritapita

2 years ago

@cyrus_tau and I are so excited to share our work on developing a CRISPR protein-guided, trans-splicing system for making versatile RNA edits!!! See the highlights in Cyrus' thread and the preprint for details!

Cyrus Tau @cyrus_tau

2 years ago

.@sitaritapita and I are excited to share RESPLICE, a new RNA-programmable mode of editing that leverages RNA targeting CRISPR proteins and the endogenous spliceosomal machinery to enable versatile RNA edits in human cells! (1/12) biorxiv.org/content/10.110…

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Nicholas Perry @ntperry13

2 years ago

Amazing week @KeystoneSymp ! So much fun to finally share our work. It’s been an awesome scientific journey characterizing this system with @mgdurrant and our team Some notes on our bridge recombination system from our preprint 🧵:

Patrick Hsu @pdhsu

2 years ago

Just shared at @KeystoneSymp a new @arcinstitute discovery of the bridge RNA recombinase mechanism: a new class of natural RNA-guided systems that retains the key property of programmability from RNAi and CRISPR while enabling large-scale genome design beyond RNA and DNA cuts

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Alison Fanton @AlisonFanton

3 years ago

@pdhsu @UofCalifornia Thank you Patrick!!☺️

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Patrick Hsu @pdhsu

5 years ago

By the way, if this flavor of molecular technology development excites you, we are hiring (at all levels)! Please reach out 👩‍💻🧑‍💻👩‍🔬👨🏻‍🔬

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Patrick Hsu @pdhsu

5 years ago

The ability of molecular biologists to control DNA has relied on enzymes derived from the phage-microbe arms race. In this new work, we systematically mined thousands of new serine integrases (LSRs) and developed them for human genome editing. 🧬 biorxiv.org/content/10.110…