Ning Zhao @NingInScience
Asst. Prof. @ CU-Anschutz | gene regulation and protein folding | single-particle tracking | live-cell imaging | she/her medschool.cuanschutz.edu/biochemistry/p… Colorado, USA Joined November 2018-
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@Steve_Bonilla @pewtrusts Congrats, Steve!
@Guiping_W @CRUK_CI Congrats! This's so exciting.
@angalus_d @cdasno Thanks, Sulagna! Let me know if you need the plasmids or sequence files.
We have developed a cysteine-free, highly thermostable tagging system, UTag, that enables single-mRNA translation tracking in live cells. You may wonder how different tagging systems affect translation kinetics—we addressed this by performing a systematic comparison.
We benchmarked UTag against SunTag and ALFA-tag for single-mRNA translation tracking and observed comparable translation spot intensity and signal-to-noise ratios. Importantly, translation kinetics were also comparable across systems, as determined by both ACF and harringtonine.
To further enhance its robust folding in intracellular environments, we engineered a cysteine-free variant with improved thermostable (Tm ~80 °C) that maintains the high UTag binding affinity. Excitingly, both intrabodies enable single-mRNA translation tracking in live cells.
New preprint from our lab, led by Luis Aguilera! We have developed an open-source software, "MicroLive," to track single molecules in live cells. This one-step platform enables researchers without coding experience to perform single-molecule studies in a user-friendly interface!
MicroLive: An Image Processing Toolkit for Quantifying Live-cell Single-Molecule Microscopy biorxiv.org/content/10.110… #biorxiv_biophys
@FrydmanLab @scafrancesco1 @DeuerlingLab We are going to discuss this paper in our journal club today. 😀
Imaging single endogenous RNA in live cells comes true!
Single-molecule live-cell RNA imaging with CRISPR–Csm go.nature.com/3EJPKsM
@EricTopol @DoudnaLab @NatureBiotech The RNA signal is so good. Beautiful work!
Visualizing RNA in live cells using CRISPR @DoudnaLab @NatureBiotech nature.com/articles/s4158…
IgGM: A Generative Model for Functional Antibody and Nanobody Design 1/ IgGM is a novel generative model designed to facilitate the de novo design of antibodies and nanobodies with functional specificity, focusing on their ability to bind specific antigens. This model uniquely generates both the sequences and structures of antibodies in a single step. 2/ The model consists of three main components: a pre-trained language model that extracts sequence features, a feature learning module to identify relevant features, and a prediction module to generate antibody sequences and predict the antibody-antigen complex structure. 3/ IgGM addresses a major challenge in antibody design by generating novel antibody sequences and their structures without the need for existing experimental antibody-antigen complex structures, making it suitable for scenarios involving new antigens. 4/ The model is capable of designing antibody CDR regions, which are crucial for antigen binding, and can also predict and design entire antibody structures, a significant advancement over existing methods that often require pre-existing structures or templates. 5/ IgGM excels in both antibody and nanobody design tasks, achieving superior performance in multiple design scenarios, such as generating antibodies with high specificity and binding affinity to antigens, outperforming previous methods in terms of sequence and structure fidelity. 6/ Its ability to design antibodies and nanobodies for specific epitopes, including the design of multiple CDR regions simultaneously, provides a versatile solution for therapeutic and diagnostic applications. 7/ Experimental results demonstrate IgGM's impressive accuracy, including higher success rates in docking performance and lower RMSD values compared to existing methods, showcasing its potential in the rapidly growing field of AI-driven drug design. 💻Code: github.com/TencentAI4S/Ig… 📜Paper: biorxiv.org/content/10.110… #AntibodyDesign #NanobodyDesign #GenerativeModels #MachineLearning #AIinHealthcare #DrugDiscovery #Bioinformatics #ComputationalBiology #DeepLearning #AIforMedicine #ProteinDesign
@jjanetzko @FaboPolanco We have interacted before. Small world, John!
@FaboPolanco Good luck with your next step, Fabian!
Emergency town hall at #BPS2025 Impact of U.S. policies on biophysics research and what you can do about it. Tuesday, February 18 1:30 pm - 3:30 pm Room 411
Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) biorxiv.org/content/10.110…
Timothy @tim_stasevich
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Lauren Cote @_lecote_
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Raluca Groza @RaluucaG
742 Followers 757 Following Postdoc in the Kleele lab, ETH Zürich | HFSP and EMBO fellow | Previously PhD in the Ewers lab, FU Berlin | mitochondria, neurons, super-resolution microscopy
Anatoly @t2v12
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Guido Papa @guidovir_
633 Followers 852 Following Group Leader at the University of Milan @LaStatale🇮🇹 and INGM Interested in viruses and Targeted Protein Degradation🧬🦠 Previous @MRC_LMB🇬🇧; @ICGEB🇮🇹
Nishant Suman @nishantsuman3
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Symplectic Manifold o... @MaxDiffusionRL
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197 Followers 4K Following Anesthesia doctors, should proceed with caution! An anesthesiologist (deputy chief physician) for 20 years in Beijing Hospital, mother of two boys, freelance
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Tina Termini @CterminiPhD
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Harmit S. Malik @HarmitMalik
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Christophe Leterrier @christlet
23K Followers 2K Following ➡︎ now at https://t.co/HBhh8WD3B0 Archive of tweets (2009-2024) at https://t.co/fJOiZP928a
Frances Arnold @francesarnold
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51K Followers 2K Following Just another LLM. Tweets do not necessarily reflect the views of people in my lab or even my own views last week. https://t.co/fZAnUCqd12
Ardem Patapoutian @ardemp
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RNA Bioscience Initia... @rnabioco
3K Followers 308 Following The RNA Bioscience Initiative at the University of Colorado Anschutz Medical Campus (@cuanschutz)
Nature Methods @naturemethods
155K Followers 1K Following Nature Methods publishes cutting-edge methods, tools, analyses, resources, reviews, news and commentary, supporting life sciences research. Tweets by editors.
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Prachee Avasthi @PracheeAC
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Rita Strack @rita_strack
21K Followers 4K Following Chief Editor of Nature Biomedical Engineering. Ph.D. Love methods for seeing the invisible. 🔬 All tweets and comments represent my independent views. (she/her)
Taekjip Ha @taekjip
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ShorterLab @ShorterLab
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Nils Walter Lab @NilsWalterLab
2K Followers 214 Following We study cool RNAs - reaching unprecedented single molecule sensitivity with fancy microscopes and answering the question of how cellular life works
David R. Liu @davidrliu
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Qing-Lin Yang 杨清�... @Burg1ess
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Wren Kim @jwrenkim
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Tho Nguyen @huuthoth
70 Followers 467 Following #Neuroscience #fly NMJ #scRNAseq # R, python, macro #Dad of 2 sons
Phyllis Hanson @PIHanson
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Jonathan Ouyang @jouyan11
69 Followers 153 Following 18 | ribosome biophysics @ jhu & jhmi | NASAT quizbowl national champion | Green Lab & Buskirk Group & Trcek Lab | building @collidetherapeu
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129 Followers 313 Following Postdoc @hansen_lab @MIT | @AmericanCancer fellow, former @AICF_nyc fellow | PhD Davide Mazza Lab @MyUniSR | 3D genome, TFs, gene regulation
Alexander Bartelt @BarteltLab
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Sunny Yadav @sunny_de_sole
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Maximillian Marin @maxgmarin
553 Followers 2K Following Researcher studying genomics and infectious disease at Harvard Medical School.
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Christos Constantinid... @c_constan
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Shahab Khan @iamsakc
246 Followers 2K Following
Ventura's Lab @PPMC_UAB
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Jason Yang @jsunn_y
824 Followers 970 Following PhD candidate @Caltech studying ML for protein engineering | @jsunn-y.bsky.social | 65% oxygen, 18% carbon, 10% illenium, 7% caesar salad | 🌈
Deuerling Lab @DeuerlingLab
488 Followers 103 Following We are interested in understanding the basic principles of cellular proteostasis under normal, aging and disease conditions. Run by lab members. @UniKonstanz
Paul Daly @paulSH02
754 Followers 3K Following Associate Professor, Jiangsu Academy of Agricultural Sciences. CAZymes, filamentous fungi, oomycetes, microbe-microbe & plant-microbe interactions, genomics.
Rui Zhang @RuiZhangWUSTL
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Ruben Gonzalez @rubenlgonzalez
2K Followers 711 Following Dean of Science, Professor of Chemistry, and Professor of Physiology & Cellular Biophysics at Columbia University | Championing Science, Learning, and Community
Sonya Neal @Neal_Lab
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CU Anschutz Dept of I... @CUAMCImmu_micro
562 Followers 230 Following Official Twitter account of the Department of Immunology & Microbiology @CUAnschutz
Hao Yin @HaoYin20
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Jorge Bravo Abad @bravo_abad
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Deen Sun @DeenSun3
432 Followers 616 Following Assistant professor @PKU1898 | Postdoc in Kai Johnsson Lab @mpi_mr_hd | Ph.D in @XingChen_PKU Lab | Chemical biology, Expansion🔬, Molecular labeling
Krishna C Suddala @krishnacs
212 Followers 507 Following Senior Scientist @Sanofi mRNA CoE, Single-molecule & RNA structural biophysicist. Postdoc @NIDDK, NIH. PhD @UMich. @IITKanpur and @VIT_univ alum.
May Khanna @may_khanna
2K Followers 4K Following Mom and scientist. @UF faculty and assistant dean of innovation and entrepreneurship. My opinions are my own and do not reflect the views of my employer. 🇺🇸
Hayato Ito @Itoh_tech
49 Followers 173 Following 🇯🇵| Tokyo tech PhD candidate in Taguchi lab | RNA biology(RAN translation, RNA binding proteins, ribosome), Neurodegenerative diseases (ALS, SCA) | 伊藤隼人
Akos Nyerges @AkosNyerges
2K Followers 996 Following #SyntheticGenomics #GenomeDesign & #DirectedEvolution | Principal Investigator at @harvardmed
Rong Fan @RongFan8
11K Followers 2K Following Harold Hodgkinson Professor of Biomedical Engineering and Pathology, Yale University. *Personal Account*. Views are my own.
christopher snow @cdasno
6 Followers 97 Following
Babak Razani, MD, PhD... @RazaniLab
1K Followers 811 Following Razani lab @ Center for Immunometabolism https://t.co/4MwIEByzBT, U. of Pittsburgh studies macrophages, autophagy-lysosomes, nutrient signaling in metabolic disease
Usman Hyder @whoishegotyou
419 Followers 273 Following I like transcription and chromatin. Currently NCI K00 Fellow with David Bentley (University of Colorado Medical). PhD with @dorsolab (UT Southwestern).
Xuebing Wu @wu_xuebing
3K Followers 2K Following Assistant Professor @Columbia University | RNA, genome, ribosome, CRISPR, AI | alum @MIT @WhiteheadInst @Tsinghua_Uni | #hiringNow
Biochemical Journal @Biochem_Journal
987 Followers 490 Following Founded in 1906, the Biochemical Journal publishes high-impact research and reviews in 21st century biochemistry, produced by @PPPublishing for the @BiochemSoc.
DeepSeek @deepseek_ai
1.0M Followers 0 Following Unravel the mystery of AGI with curiosity. Answer the essential question with long-termism.
Debo (Debosmita Sarda... @debo_Astrocyte
715 Followers 365 Following Assistant Professor @CUAnschutz @CUPharmacology All things glia, epigenetics, and olfaction.. https://t.co/Dp0TSyuuTE
Pengning Xu @XPengning
55 Followers 346 Following current postdoc Hahn lab @UNC: single particle live cell Cdc42 biosensor; Phd Weninger lab @NCSU: DNA repeat structures/dynamics with smFRET; flew to bluesky
David Balchin @balchin_david
787 Followers 632 Following Biochemist interested in protein biogenesis. Group leader @TheCrick.
Niko Dalheimer @NikoDalheimer
219 Followers 679 Following PhD student in F.U.Hartl group | @MPI_Biochem | Molecular Cell Biology | Single Particle Tracking | Proteostasis | Protein misfolding
Rebecca Voorhees @voorheeslab
2K Followers 472 Following Assistant professor of biology @caltech | HHMI Freeman Hrabowski Scholar @HHMINEWS | membranes, microscopes 🔬❄️, mitochondria | she/her #cubana
König Lab @koenig_lab
1K Followers 537 Following RNA biology group @Uni_WUE & formerly @IMBMainz Run by lab members 🧬 #Splicing #RNAModifications #RBPs #iCLIP #miCLIP #RQC
Martin Breuss @BrEUssMartin
142 Followers 129 Following Assistant Professor in Genetics Interested in genomic mosaicism and neurodevelopment. Opinions are my own.
Breuss Lab @BreussLab
40 Followers 30 Following Laboratory of Genomic and Cellular Mosaicism at University of Colorado Anschutz Medical Campus, Department of Pediatrics | Genetics and Metabolism
Angelo D'Alessandro @dalessandrolab
803 Followers 312 Following Disclaimer: Opinions expressed here are my own, do not represent my lab, employer, or funding agencies
Stefan W. Hell @Stefan_W_Hell
4K Followers 36 Following Physicist, Nobel Laureate in Chemistry, Father of four
Yongsheng Shi @YongshengShi
1K Followers 186 Following Biologist at @UCIrvine, #RNA #stemcell #virology

















