AJ Sethi @_AJSethi

SWARM (Single-molecule Workflow for Analysing RNA Modifications) detects pseudouridine, m6A, and m5C on individual molecules from direct RNA nanopore signals (in both chemistries RNA002 and RNA004) github.com/comprna/SWARM see more on poster P2-396 at #rna2025 #RNA25 #RNAmods

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AJ Sethi @_AJSethi

a year ago

Thanks to all co-authors, without whom this work would be impossible. @GuarnacciMarco @starstuffkarthi @pol2rna @TominAustralia @RippeiH and others.

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AJ Sethi @_AJSethi

a year ago

dFORCE is directly applicable to mammalian tissue samples, and produces results consistent with cell culture, unlocking analysis of mRNA synthesis kinetics in vivo:

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AJ Sethi @_AJSethi

a year ago

We describe the temporal organisation of central pre-mRNA processing events, including splicing, m6A modification and 3' end formation, during mRNA synthesis. Check out the preprint here!

Eduardo Eyras @EduEyras

a year ago

"Single-molecule multimodal timing of in vivo mRNA synthesis" a collaboration with Rippei Hayashi's lab, work led by AJ Sethi @_AJSethi #RNA #premRNA #splicing #RNAprocessing #m6A #polyadenylation biorxiv.org/content/10.110…

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AJ Sethi @_AJSethi

2 years ago

@ItaiYanai

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AJ Sethi @_AJSethi

2 years ago

it gets me fr

Linus ✦ Ekenstam @LinusEkenstam

2 years ago

Ask ChatGPT “based on what you know about me. draw a picture of what you think my current life looks like” past your responses below. thanks again @mreflow & @danshipper

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Eduardo Eyras @EduEyras

2 years ago

R2Dtool github.com/comprna/R2Dtool for handling, integrating, and viualising isoform-resolved RNA feature data - led by @_AJSethi #bioinformatics #RNA #isoforms academic.oup.com/bioinformatics…

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AJ Sethi @_AJSethi

2 years ago

@ribonucleicacid The spliced AS transcripts could also be RT artefacts. I don't see almost any antisense mRNA reads in cerevisiae nanopore RNA libraries.

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AJ Sethi @_AJSethi

2 years ago

@ribonucleicacid And a little further down at RPS18B:

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AJ Sethi @_AJSethi

2 years ago

@ribonucleicacid I'm seeing splicing of sense introns on the antisense transcripts, which isn't possible if these transcripts arise from genomic transcription. Could the AS transcripts come from RNA-dependant RNA polymerase transcription of processed mRNA? RPS17A in SRR27483326 shown: