FISchmidtLab @FISchmidtLab
Cell Biology meets Immunology and Virology, spiced up with nanobodies tinyurl.com/FISchmidtLabor… Bonn, Germany Joined May 2018-
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Late #WeekendRead: We describe a novel reporter to visualize endogenous #inflammasome assembly. Suitable for cell lines, primary cells, and tissues as well as quantification by #microscopy and #flowcytometry. Happy to share. biorxiv.org/content/10.110…
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FISchmidtLab retweeted
How could we identify key genes involved in any biological process and cell type? Please read on about Nuclear In-Situ Sequencing (NIS-Seq) nature.com/articles/s4158…
@IpbsToulouse @MariaMMota2 @LennonLab This was truly a lot of fun - excellent talks at @IpbsToulouse (and was also spoiled with good food). Thanks to all the students and staff members for organizing this!
FISchmidtLab retweeted
and so it begins.....science needs to start getting serious about how how to deal with this type of attacks, sowing distrust.
RFK Jr. Dismantles Journalist's Appeal to Authority Journalist: "You're a lawyer and activist, not a doctor or scientist." RFK Jr.: "Relying on experts is akin to religious faith and totalitarianism. In a democracy, we question everyone."
Check out Lisa‘s take on her recent paper on the mechanism (and potential therapeutic targeting) of #GSDMD pore formation using #nanobodies.
🎬 Episode 11 of #SpotOnScience is out! In this episode, Lisa Schiffelers from the group of @FISchmidtLab shares insights into her work on unraveling gasdermin D (GSDMD) pore formation using nanobodies. 👩🔬 Check out the episode: youtube.com/watch?v=HvgNf5…
FISchmidtLab retweeted
It is out! #NanoPlex is the universal strategy for multiplexing any immunofluorescence using erasable #nanobodies from @_NanoTag_. We showcase easy multiplexed STED, STORM, and 21-plex Confocal #Microscopy. Published in @NatureComms here: rdcu.be/dWvzO
FISchmidtLab retweeted
😯😯
It is out! #NanoPlex is the universal strategy for multiplexing any immunofluorescence using erasable #nanobodies from @_NanoTag_. We showcase easy multiplexed STED, STORM, and 21-plex Confocal #Microscopy. Published in @NatureComms here: rdcu.be/dWvzO
FISchmidtLab retweeted
Teaching the next generation of scientists all about what we do in the lab #TürenaufmitderMaus – big thank you to my whole lab and, in particular, Fabian @IIIBonn @for5547 #sfb1454 @ImmunoSens
FISchmidtLab retweeted
Epic effort from Flo & first author Lisa Schiffelers of @FISchmidtLab . Antagonistic camelid #nanobodies are small enough to traverse early #GSDMD pores to inhibit further #pore #formation. Monomers target the membrane, suggesting #monomer to #oligomer transition on #membrane.
Ever wondered why there is so little #microscopy of #GSDMD pores? Check out how Lisa Schiffelers used #Nanobodies to stabilize monomeric GSDMD in the plasma membrane, to dissect the steps of pore formation, and to develop some new ideas for therapy. nature.com/articles/s4146…
In parellel work, @anja_kopp @Geyer_lab solved the structure of two of our #nanobodies bound to #GSDMD. nature.com/articles/s4146…. The Feng Shao lab also used #AAV to express one of our #GSDMD #nanobodies to prevent loss of the #BBB during sepsis. nature.com/articles/s4158…
Special thanks to all the lab members who helped during the revision, including @YM_Tesfamariam, Lea, Stefan, Sabine and Steffen. This work was done at @IIIBonn @UniBonn @UniklinikBonn with funding by @ImmunoSens @sfb_1403 @237Crc .
We think the antagonistic GSDMD #nanobodies enter cells when the first #GSDMD pores form. They prevent any further pore formation and thus -in combination with cellular repair mechanism- prevent cell death by pyroptosis. We are looking forward to test this concept in vivo ...
As #nanobodies enter pyroptotic cells (left), Lisa also tested if recombinant #nanobodies prevent pyroptosis when added to the medium during #inflammasome activation (right). They do!
As cells no longer die by #pyroptosis, stabilized, endogenous #GSDMD in the plasma membrane can even stained with antibodies for cleaved GSDMD.
Surprisingly, expression of antagonistic #GSDMD #nanobodies stabilized monomeric GSDMD NTD-mNeonGreen in the plasma membrane.
We had previously tried to use a cool tool from @inflammgenetics to visualize #GSDMD pores in living cells (mNeonGreen remains with the #GSDMD NT), but mostly found GSDMD in intracellular structures ...
We raised #alpaca #nanobodies against #GSDMD and found that two cytosolically expressed nanobodies completely shut down pore formation, #pyroptosis, and cytokine release after #inflammasome activation ....
Ever wondered why there is so little #microscopy of #GSDMD pores? Check out how Lisa Schiffelers used #Nanobodies to stabilize monomeric GSDMD in the plasma membrane, to dissect the steps of pore formation, and to develop some new ideas for therapy. nature.com/articles/s4146…
FISchmidtLab retweeted
✨Today officially marks 10 years of @TheDohertyInst. From pioneering research on the immune system to our response to COVID-19, we’re incredibly proud of our staff and students and their achievements in infection and immunity. And a huge thank you to our supporters. Here’s to another 10 years and beyond! go.unimelb.edu.au/2cc8 @UniMelbMDHS @TheRMH @UniMelb
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@LabWilhelm
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